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KMID : 0366220050400020075
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Korean Journal of Hematology
2005 Volume.40 No. 2 p.75 ~ p.81
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Apoptosis Induction by Curcumin in Human Myelogenous Leukemia Cell Lines
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Cho Yo-Han
Kim Young-Ju
Park Ju-Won
Ahn Kwang-Sung
Kim In-ho
Bae Eun-Kyung
Park Seon-yang
Kim Byoung-Kook
Yoon Sung-Soo
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Abstract
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Background: Curcumin, a natural, biologically active compound extracted from rhizomes of Curcuma species, has been shown to possess potent anti-inflammatory, anti-tumor and anti-oxidative properties. We investigated effects and possible mechanism of this agent on 2 human myelogenous leukemic cell lines.
Methods: K562 and KG-1 cells were selected. MTT assay was used for cytotoxicity and flow cytometry for cell cycle analysis. Protein expressions were analyzed by Western blotting and caspase activity was checked.
Results: Two cell lines showed dose-dependent susceptibility to curcumin. Cell cycle analysis showed increased sub-G1 phase in KG-1 cell. In K562 cell, PCNA (proliferating cell nuclear antigen) and cyclin D1 and B1 were down regulated by curcumin. Akt was also down-regulated and caspase-3 was activated to induce cleaved PARP (polyadenosine ribose polymerase) and apoptosis. However, phospho-Erk was not affected. Co-treatment of cyclosporin A (CsA) with curcumin resulted in attenuation of apoptosis in K562 cell, implying that curcumin-induced apoptosis depends on the cytochrome c release from the mitochondria.
Conclusion: Curcumin induced cell cycle arrest and apoptosis in human myelogenous leukemic cell lines and the apoptosis seemed to be dependent on the release of cytochrome c from the mitochondria.
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KEYWORD
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Curcumin, Myelogenous leukemia, Apoptosis
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